Monoclonal anti-TL antibody recognizing a TL.2-like antigen

We obtained a monoclonal anti-TL antibody-producing hybridoma by fusion of Sp2/0 hybrid cells with spleen cells from (B6 X A-Tlab) F1 mice immunized with ASL1 leukemia cells. The antibody, TL-22-27-17, reacts in complement-dependent cytotoxicity assay with ASL1 cells as well as with normal thymocytes of TL+ mouse strains (B6-Tlaa, A, BALB/c, DBA/2 and 129) but not with thymocytes from TL- mice (B6--Ly-1a). In addition, it did not react with lymph node cells or Concanavalin A-induced spleen blasts from B6-Tlaa mice; this result shows that TL-22-27-17 recognizes TL and not Qa-1 antigen. The positive reactions with BALB/c (TL.1-2+3-5-6-7+) and 129 (TL.1-2+3-5-6-7-) thymocytes suggest that antibody TL-22-27-17 reacts with TL.2 or TL.2-like antigenic determinant.