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Related Experiment Videos

Granulocyte collection by continuous-flow centrifugation using arteriovenous shunts.

B M Williams, R A Clift, C D Buckner

    Journal of Clinical Apheresis
    |January 1, 1984
    PubMed
    Summary

    Continuous-flow centrifugation for granulocyte collection using Aminco Celltrifuge I, Fenwal Celltrifuge II, and IBM 2997 showed varying yields. Hydroxyethyl starch improved collection, while daily collections decreased granulocyte yield.

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    Epidemiology and infection·2022

    Area of Science:

    • Hematology
    • Transfusion Medicine

    Background:

    • Granulocyte transfusions are critical for treating infections in immunocompromised patients.
    • Continuous-flow centrifugation is a common method for apheresis, including granulocyte collection.

    Purpose of the Study:

    • To evaluate the efficacy of different continuous-flow centrifuges for granulocyte collection.
    • To identify factors influencing granulocyte collection yield and donor safety.

    Main Methods:

    • Analysis of 2,927 granulocyte collections using Aminco Celltrifuge I, Fenwal Celltrifuge II, and IBM 2997.
    • Assessment of hydroxyethyl starch infusion and impact of repeated daily collections on yield and donor health.

    Main Results:

    • Fenwal Celltrifuge II yielded smaller granulocyte collections compared to Celltrifuge I and IBM 2997.

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  • Hydroxyethyl starch infusion increased granulocyte collection efficiency.
  • Repeated daily collections led to anemia in donors, necessitating red cell transfusions.

    • Conclusions:

    • Centrifuge type impacts granulocyte collection yield; Celltrifuge II was less effective.
    • Hydroxyethyl starch is a beneficial adjunct for enhancing granulocyte collection.
    • Donor safety is a concern with repeated granulocyte donations, particularly regarding anemia.