Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Microcapsules on Streptococcus mutans serotypes by electron microscopy.

E M Grenier, R H Gray, W J Loesche

    Journal of Dental Research
    |February 1, 1977
    PubMed
    Summary
    This summary is machine-generated.

    Related Concept Videos

    You might also read

    Related Articles

    Articles linked to this work by shared authors, journal, and citation graph.

    Sort by
    Same author

    The effects of combinations of antibiotics on Clostridia in vitro.

    Antibiotics & chemotherapy (Northfield, Ill.)·2014
    Same author

    The status, distribution and ecology of wildlife on the U.S. Doe Hanford Site: A historical overview of research activities.

    Environmental monitoring and assessment·2013
    Same author

    Past and present water-quality conditions in the Hanford Reach, Columbia River.

    Environmental monitoring and assessment·2013
    Same author

    Multimedia environmental monitoring: 50 years at Hanford.

    Environmental monitoring and assessment·2013
    Same author

    Immune correlates of HIV exposure without infection in foreskins of men from Rakai, Uganda.

    Mucosal immunology·2013
    Same author

    Rediscovering Sig Socransky, the genius and his legacy.

    Journal of dental research·2012

    Extracellular microcapsules on Streptococcus mutans correlate with antigenicity. Loss of these capsules and antigenicity in vitro was reversible with rabbit serum, suggesting a link between capsule and immune response.

    Area of Science:

    • Microbiology
    • Immunology

    Background:

    • Streptococcus mutans is a key cariogenic bacterium.
    • Extracellular microcapsules play a role in bacterial virulence and host interactions.

    Purpose of the Study:

    • To investigate the presence and role of extracellular microcapsules in Streptococcus mutans.
    • To determine the relationship between microcapsule presence and bacterial antigenicity.

    Main Methods:

    • Electron microscopy was used to visualize extracellular microcapsules on various Streptococcus mutans serotypes.
    • Immune serum (peroxidase-labeled or unlabeled) was used for detection.
    • Indirect fluorescent antibody (FA) testing assessed bacterial antigenicity.
    • In vitro passage and addition of rabbit serum were used to study capsule and antigenicity changes.

    Related Experiment Videos

    Main Results:

    • Extracellular microcapsules were observed on most Streptococcus mutans serotypes.
    • A positive correlation was found between the amount of capsular substance and antigenicity (FA title).
    • A serotype d strain lost both antigenicity and capsular material after repeated in vitro passage, which was restored by adding unheated rabbit serum.

    Conclusions:

    • Extracellular microcapsules on Streptococcus mutans are linked to bacterial antigenicity.
    • Capsular material and antigenicity can be modulated in vitro, with rabbit serum promoting their restoration.