Concentration of blood coagulation factors by ultrafiltration in hollow fiber artificial kidney

We have concentrated plasma coagulation factors by a process of ultrafiltration (UF) using a hollow fiber artificial kidney with applied negative pressure. The coagulation factors were concentrated by between 2.4 and 3.2 times, resulting in yields of 82-100%. There was no evidence of activation of coagulation factors as shown by approximately equal increases in the concentrations of all three moieties of factor VIII following ultrafiltration, and by the fact that plasma recalcification times post-UF were not increased. It is suggested that the concentrated plasma product from UF may be useful in replacement therapy.