[Improved method of ectopic transplantation of the whole spleen in mice]

A simple and reliable technique has been developed for transplantation of the whole mouse spleen on the kidney. This technique ensures sufficient structural and functional recovery (transfer) of the original splenic microenvironments with a survival rate of 100%. The method allows counting the antibody-producing cells, as well as the spleen colony-forming units on the ectopic splenic territories. It may be used for studying hemopoietic and lymphoid microenvironments or for other special purposes.