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A murine model for bladder cancer.

G P Murphy, A A Sandberg, J E Pontes

    Progress in Clinical and Biological Research
    |January 1, 1984
    PubMed
    Summary
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    This study introduces a mouse bladder cancer model for studying invasive tumors. While Cis-diaminedichloroplatinum II (DDP) slowed tumor growth, Cyclophosphamide (CTX) significantly improved survival in this model.

    Area of Science:

    • Oncology
    • Cancer Research
    • Translational Medicine

    Background:

    • Transplantable bladder cancer models are crucial for preclinical research.
    • N-[4-5-nitro-2-furyl)-2-thiazolyl] formamide (FANFT) induces transitional cell carcinoma in mice.
    • Understanding tumor growth and metastasis is key to developing effective treatments.

    Purpose of the Study:

    • To characterize a transplantable mouse bladder tumor model induced by FANFT.
    • To evaluate the efficacy of chemotherapy agents in this model.
    • To assess the model's suitability for studying locally invasive bladder cancer.

    Main Methods:

    • Subcutaneous implantation of FANFT-induced transitional cell carcinoma in mice.
    • Monitoring of survival time, tumor burden, and metastasis.

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  • Histological and cytogenetic analysis of primary tumors and metastases.
  • Evaluation of chemotherapy drugs: Cis-dichloro-trans-dihydroxy-bis-iso propylamine platinum IV (CHIP), Cis-diaminedichloroplatinum II (DDP), Cyclophosphamide (CTX), and Methotrexate (MTX).

    • Main Results:

    • Mice survived a mean of 43 ± 7 days with an average tumor burden of 8.45 ± 0.60 gm.
    • Undifferentiated anaplastic tumors with spindle cells observed in primary and metastatic sites.
    • Significant decrease in DNA synthesis noted between 13 and 20 days post-implantation.
    • DDP significantly retarded tumor growth (p < 0.005), but did not improve survival.
    • CTX significantly improved survival (p < 0.001) without significantly reducing tumor growth.
    • Lung metastasis occurred in all treatment groups.

    Conclusions:

    • The FANFT-induced mouse bladder tumor model exhibits characteristics suitable for studying locally invasive bladder cancer.
    • DDP and CTX show differential effects on tumor growth and survival, highlighting the need for tailored therapeutic strategies.
    • The model facilitates the evaluation of chemotherapeutic agents and provides insights into tumor progression and metastasis.