Subacute apomorphine injections in rats: effects on components of behavioral stereotypy

This study was aimed at documenting the changes in the frequency and duration of bouts of behavior of Sprague-Dawley male rats in the open-field following each of four injections of apomorphine (Apo, 5 mg/kg, sc, immediate), or normal saline, delivered at 3-day intervals. Independent quantification of locomotion, sniffing, rearing, grooming, inactivity, gnawing, nodding, and jumping was obtained continuously throughout the 78-min sessions. Apo eliminated grooming and inactivity on all sessions. The large increases in locomotion and sniffing seen in the Apo rats compared to the saline rats on the first session were sustained throughout subsequent sessions. However, the Apo-induced potentiation of nodding of the head and gnawing, seen acutely, declined across sessions. These observations reconcile inconsistencies in the literature on subacute Apo effects. Finally, the individual differences in behavioral scores of Apo-treated rats were more stable than were those of saline-treated rats. This finding supports evidence in the literature that individual differences in neurochemistry are more likely to be predicted from the behavioral scores of Apo-challenged rats than from the scores of untreated rats.