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Cellular retinol-binding protein. Quantitation and distribution.

U Eriksson, K Das, C Busch

    The Journal of Biological Chemistry
    |November 10, 1984
    PubMed
    Summary
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    Cellular retinol-binding protein is abundant in tissues crucial for vitamin A transport, including the liver, jejunum, and kidney. Its levels and distribution remain unaffected by vitamin A status, suggesting a key role in retinol intracellular transport.

    Area of Science:

    • Biochemistry
    • Cell Biology
    • Nutritional Science

    Background:

    • Cellular retinol-binding protein (CRBP) plays a role in vitamin A metabolism.
    • Understanding CRBP distribution is crucial for elucidating vitamin A transport mechanisms.

    Purpose of the Study:

    • To localize cellular retinol-binding protein (CRBP) in key vitamin A transport tissues.
    • To quantify CRBP levels and assess the impact of retinol status on its distribution.

    Main Methods:

    • Peroxidase-antiperoxidase technique for protein localization.
    • Radioimmunoassay for quantitative analysis of CRBP levels.

    Main Results:

    • CRBP was localized in hepatocytes and fat-storing cells of the liver, jejunal epithelial cells, and proximal tubule cells.

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  • Retinol deficiency did not alter CRBP tissue levels or subcellular distribution.
  • High CRBP concentrations were observed in cells involved in vitamin A uptake, storage, mobilization, and resorption.

    • Conclusions:

    • Cellular retinol-binding protein (CRBP) is strategically distributed in tissues critical for vitamin A transport.
    • CRBP likely functions as an intracellular vehicle for retinol transport within these organs.