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Studies on simian hemorrhagic fever virus nucleocapsids
Archives of Virology
|January 1, 1984
Summary
This study characterizes Simian Hemorrhagic Fever (SHF) virus, revealing its nucleic acid content and sedimentation properties. Purified SHF virions exhibit a 214S sedimentation coefficient, with nucleocapsids showing RNAse sensitivity.
Area of Science:
- Virology
- Molecular Biology
- Biophysics
Background:
- Simian Hemorrhagic Fever (SHF) is a significant viral disease affecting non-human primates.
- Understanding the biophysical properties of SHF virions is crucial for developing effective countermeasures.
Purpose of the Study:
- To determine the sedimentation coefficient of purified Simian Hemorrhagic Fever virions.
- To analyze the nucleic acid content and characteristics of SHF virions and their nucleocapsids.
Main Methods:
- Purification of Simian Hemorrhagic Fever virions.
- Ultracentrifugation in neutral sucrose gradients for sedimentation analysis.
- Analysis of nucleocapsids after nonionic detergent treatment.
- Buoyant density determination in cesium chloride gradients.
Main Results:
- Purified SHF virions displayed a sedimentation coefficient of 214S.
- Nucleic acid constituted approximately 8% of the total virionic mass.
- Isolated nucleocapsids were RNAse-sensitive but DNAse-resistant, with a sedimentation coefficient of 174S and a buoyant density of 1.33 g/ml in CsCl.
Conclusions:
- The biophysical characterization of Simian Hemorrhagic Fever virions provides essential data for understanding its structure.
- The findings confirm the viral RNA nature and provide a basis for further molecular studies of SHF virus.