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Phospholipids of Golgi subfractions determined with an enzymatic method
Experimental Pathology
|January 1, 1984
Abstract:
Golgi apparatus subfractions are still under consideration both for morphological, biochemical and functional characterization. In this note, we determined glycerophospholipids and Golgi subfractions from rat liver by a method that requires the complete enzymatic hydrolysis of phospholipids with phospholipase C from CL perfringens. The main advantages of this procedure are that small amounts of protein membranes are required, the precision and the rapidity.