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Experimental cell-mediated interstitial nephritis induced with exogenous antigens
Journal of Immunology (Baltimore, Md. : 1950)
|February 1, 1977
Summary
Intrarenal injections of aggregated bovine gamma-globulin (BGG) trigger hypersensitivity reactions in guinea pigs and rats. These reactions involve mononuclear cells and are transferable via lymph node cells, not serum.
Area of Science:
- Immunology
- Nephrology
- Cellular Biology
Background:
- Hypersensitivity reactions can affect kidney function.
- The role of aggregated proteins in eliciting renal inflammation is not fully understood.
Purpose of the Study:
- To investigate the capacity of bovine gamma-globulin (BGG) to induce hypersensitivity reactions within the kidney.
- To characterize the cellular infiltrate and transferability of these renal reactions.
Main Methods:
- Intrarenal injections of aggregated or soluble bovine gamma-globulin (BGG) were administered to immunized guinea pigs and rats.
- Histopathological analysis characterized the inflammatory infiltrate.
- Transfer experiments using lymph node cells and serum were conducted in rats.
Main Results:
- Heat-aggregated BGG induced inflammatory reactions in the renal cortex of guinea pigs, while soluble BGG did not.
- Aggregated BGG was effective in eliciting reactions in rats.
- The observed reactions were characterized by a predominantly mononuclear cell infiltrate.
- Reactivity was transferable with lymph node cells but not with serum in rats.
Conclusions:
- Aggregated BGG is capable of eliciting intrarenal hypersensitivity reactions.
- These reactions are mediated by cellular components, specifically transferable via lymph node cells.
- The findings contribute to understanding immune-mediated kidney inflammation.