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The nature and function of granulopoietic microenvironments
Blood Cells
|January 1, 1984
Summary
Fibroblastic reticulum cells in bone marrow and spleen regulate granulopoiesis by producing colony-stimulating activity (CSA). Macrophages then deplete this CSA, highlighting a complex cellular interaction in hematopoietic stroma.
Area of Science:
- Hematology
- Immunology
- Cell Biology
Background:
- Fibroblastic reticulum cells in hematopoietic stroma are implicated in granulopoiesis regulation.
- Histological studies show associations between granulopoiesis and these cells in bone marrow and spleen.
Purpose of the Study:
- To identify and characterize cells involved in granulopoiesis regulation.
- To investigate the role of fibroblastic reticulum cells in producing colony-stimulating activity (CSA).
Main Methods:
- Developed a monoclonal antibody (mAb ER-HR1) targeting murine fibroblastic reticular cells.
- Utilized in vitro cultures of primary bone marrow fibroblasts and macrophages.
- Assayed for colony-stimulating activity (CSA) production and depletion.
Main Results:
- mAb ER-HR1 specifically detected fibroblastic reticular cells in murine spleen and bone marrow.
- Primary bone marrow fibroblasts produced CSA, while macrophages did not produce CSA but depleted it.
- These findings support a regulatory role for fibroblastic reticulum cells in granulopoiesis.
Conclusions:
- Fibroblastic reticulum cells possess unique antigenic determinants and contribute to granulopoiesis regulation.
- A complex cellular network involving fibroblasts, macrophages, endothelial cells, and lymphocytes controls the hematopoietic microenvironment.
- Fibroblastic cells are likely the source of CSA in bone marrow cultures, with macrophages playing a role in its regulation.