Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

A radioimmunoassay for bleomycin.

M K Elson, M M Oken, R B Shafer

    Journal of Nuclear Medicine : Official Publication, Society of Nuclear Medicine
    |March 1, 1977
    PubMed
    Summary
    This summary is machine-generated.

    Related Concept Videos

    You might also read

    Related Articles

    Articles linked to this work by shared authors, journal, and citation graph.

    Sort by
    Same author

    Selection of Microorganisms for Biological Control of Silver Scurf (Helminthosporium solani) of Potato Tubers.

    Plant disease·2019
    Same author

    Interferon in the Treatment of Refractory Multiple Myeloma: An Eastern Cooperative Oncology Group Study.

    Leukemia & lymphoma·2016
    Same author

    Influence of antifungal compounds from a soil-borne actinomycete onFusarium spp. in asparagus.

    Journal of chemical ecology·2013
    Same author

    Oral antibiotic prophylaxis of early infection in multiple myeloma: a URCC/ECOG randomized phase III study.

    Leukemia·2012
    Same author

    A phase II trial of melphalan or thioguanine in the treatment of patients with advanced renal cell cancer.

    Urologic oncology·2011
    Same author

    Clinical and biological significance of RAS mutations in multiple myeloma.

    Leukemia·2008
    Same journal

    Overall Survival with [<sup>177</sup>Lu]Lu-PSMA-617 Versus [<sup>177</sup>Lu]Lu-PSMA I&T: A Propensity Score-Matched Real-World Analysis.

    Journal of nuclear medicine : official publication, Society of Nuclear Medicine·2026
    Same journal

    Toward a Biopsy-Free Diagnosis of Prostate Cancer: Potential of Combined <sup>18</sup>F-Flotufolastat PSMA PET and mpMRI.

    Journal of nuclear medicine : official publication, Society of Nuclear Medicine·2026
    Same journal

    PSMA PET/CT-Targeted Biopsy in Men with Negative or Equivocal Multiparametric MRI and Exploratory Dynamic Total-Body PET: The FUPERMAN Study.

    Journal of nuclear medicine : official publication, Society of Nuclear Medicine·2026
    Same journal

    Erratum.

    Journal of nuclear medicine : official publication, Society of Nuclear Medicine·2026
    Same journal

    Live from 2026 SNMMI Annual Meeting in Los Angeles!

    Journal of nuclear medicine : official publication, Society of Nuclear Medicine·2026
    Same journal

    CAR T-Cell Therapy for Cancer: Updates and Challenges for Response Assessment.

    Journal of nuclear medicine : official publication, Society of Nuclear Medicine·2026
    See all related articles

    A new radioimmunoassay accurately measures bleomycin drug levels in serum and urine. This precise method is valuable for therapeutic drug monitoring during bleomycin treatment.

    Area of Science:

    • Pharmacology
    • Analytical Chemistry
    • Clinical Chemistry

    Background:

    • Bleomycin is an important chemotherapy agent.
    • Therapeutic drug monitoring of bleomycin is crucial for optimizing efficacy and minimizing toxicity.
    • Existing methods for bleomycin quantification may have limitations.

    Purpose of the Study:

    • To develop and validate a sensitive and specific radioimmunoassay (RIA) for quantifying bleomycin in biological samples.
    • To establish a reliable method for monitoring bleomycin serum and urine levels in patients.

    Main Methods:

    • Development of antisera by immunizing rabbits with a bleomycin-human serum albumin conjugate.
    • Use of 57Co-labeled bleomycin as a radiotracer.
    • Separation of antibody-bound and free bleomycin using polyethylene glycol precipitation.

    Related Experiment Videos

  • Validation of assay sensitivity, precision, and specificity.

    • Main Results:

    • The developed radioimmunoassay demonstrated high sensitivity, quantifying bleomycin down to 0.025 mug/ml.
    • The assay exhibited excellent precision, with a coefficient of variation of 2.8%.
    • Specificity was confirmed, as other commonly co-administered drugs did not interfere with the assay.

    Conclusions:

    • A robust radioimmunoassay for bleomycin has been successfully developed.
    • This assay is suitable for routine monitoring of bleomycin concentrations in serum and urine.
    • The method provides a valuable tool for optimizing bleomycin therapy and patient management.