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Light-regulated gene expression during maize leaf development.

T Nelson, M H Harpster, S P Mayfield

    The Journal of Cell Biology
    |February 1, 1984
    PubMed
    Summary
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    Light influences maize leaf development by controlling mRNA accumulation levels, not initial synthesis. Protein accumulation generally follows mRNA, but light is essential for light-harvesting chlorophyll a/b-binding protein (LHCP) accumulation.

    Area of Science:

    • Plant Molecular Biology
    • Photosynthesis Research
    • Maize Genetics and Development

    Background:

    • Understanding gene expression regulation is crucial for plant development.
    • Light plays a significant role in modulating photosynthetic gene expression in plants.
    • Maize leaf development involves complex transcriptional and translational regulation of key photosynthetic enzymes.

    Purpose of the Study:

    • To determine the light-dependent and light-independent expression schedules of key photosynthetic genes in maize leaves.
    • To compare the mRNA and protein accumulation patterns for ribulose 1,5-bisphosphate carboxylase (RuBPCase), phosphoenolpyruvate carboxylase (PEPCase), and light-harvesting chlorophyll a/b-binding protein (LHCP).
    • To investigate the quantitative effects of light and darkness on the synthesis and accumulation of these proteins and their corresponding mRNAs.

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    Main Methods:

    • Quantification of messenger RNA (mRNA) levels using hybridization with cloned probes.
    • Measurement of protein levels via immunodetection on protein gel blots.
    • Analysis of gene expression patterns during maize leaf development under both light and dark conditions.

    Main Results:

    • Initial synthesis of all four mRNAs (RuBPCase subunits, PEPCase, LHCP) is light-independent, with illumination affecting only accumulation levels.
    • Protein synthesis for RuBPCase and PEPCase is also light-independent but quantitatively modulated by light; LHCP protein accumulation strictly requires light.
    • Protein accumulation generally mirrors mRNA accumulation in the light, with higher protein-to-mRNA ratios observed for PEPCase and RuBPCase during dark growth.

    Conclusions:

    • Light primarily regulates the accumulation of photosynthetic gene transcripts and proteins in developing maize leaves, rather than their initial synthesis.
    • Differential light requirements exist for the accumulation of specific proteins, with LHCP being obligately light-dependent.
    • Distinct regulatory mechanisms govern protein-to-mRNA ratios, suggesting post-transcriptional or translational control influenced by light conditions.