Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Uptake of ascorbic acid by human granulocytes.

U Moser, F Weber

    International Journal for Vitamin and Nutrition Research. Internationale Zeitschrift Fur Vitamin- Und Ernahrungsforschung. Journal International De Vitaminologie Et De Nutrition
    |January 1, 1984
    PubMed
    Summary

    Human granulocytes actively accumulate ascorbic acid (vitamin C). Cell stimulation significantly boosts this uptake, suggesting a shared transport mechanism with glucose.

    Related Concept Videos

    You might also read

    Related Articles

    Articles linked to this work by shared authors, journal, and citation graph.

    Sort by
    Same author

    Lenvatinib as salvage therapy in advanced and progressive GI-NET.

    Endocrine-related cancer·2025
    Same author

    Fecal calprotectin and platelet count predict histologic disease activity in pediatric ulcerative colitis: results from a projection-predictive feature selection.

    European journal of pediatrics·2024
    Same author

    Usability and preliminary effectiveness of an app-based physical activity and education program for people with hip or knee osteoarthritis - a pilot randomized controlled trial.

    Arthritis research & therapy·2024
    Same author

    Manifestation of acute appendicitis as known but paradox visceral side effect of ulcerative colitis anti-inflammatory therapy with januskinase-inhibitor Tofacitinib (Xeljanz™).

    Pathology, research and practice·2023
    Same author

    [Hemithyroidectomy or total thyroidectomy for low-risk papillary thyroid cancer? : Surgical criteria for primary and secondary choice of treatment in an interdisciplinary treatment concept].

    Chirurgie (Heidelberg, Germany)·2022
    Same author

    [Reddish painless rapidly growing tumor of the upper eyelid with a smooth surface: Merkel cell carcinoma].

    Die Ophthalmologie·2022

    Area of Science:

    • Biochemistry
    • Cell Biology
    • Immunology

    Background:

    • Ascorbic acid (vitamin C) is essential for human health.
    • Granulocytes play a crucial role in the immune system.
    • Understanding nutrient uptake mechanisms in immune cells is important for immune function.

    Purpose of the Study:

    • To investigate the uptake kinetics of ascorbic acid in isolated human granulocytes.
    • To determine the influence of various factors (temperature, stimulants, sugars) on ascorbic acid uptake.
    • To elucidate the transport mechanism of ascorbic acid in human granulocytes.

    Main Methods:

    • Incubation of isolated human granulocytes with varying concentrations of ascorbic acid.
    • Assessment of uptake rates at different temperatures (0°C to 40°C).
    • Evaluation of the effects of N-formylated peptides, glucose, fructose, galactose, and phlorizin on uptake.

    Main Results:

    • Ascorbic acid uptake is temperature-dependent, with optimal uptake at 40°C and no uptake at 0°C.
    • N-formylated peptides significantly enhance ascorbic acid uptake, while non-formylated peptides have no effect.
    • Glucose strongly inhibits ascorbic acid uptake (Ki = 3.7 mM, 1:1 stoichiometry), similar to phlorizin's effect on glucose transport.

    Conclusions:

    • Human granulocytes actively accumulate ascorbic acid.
    • Stimulated granulocytes exhibit a marked increase in ascorbic acid uptake.
    • The transport mechanism for ascorbic acid in granulocytes likely involves the glucose transporter system.

    Related Experiment Videos