[A method for determination of prostaglandins in human seminal fluid]

Human seminal fluid has been shown to contain mainly eleven different prostaglandins. Methods for quantitative determination of prostaglandin E and 19-OH prostaglandin E are described. The methods include extraction with acetone and alkali treatment. After alkali treatment prostaglandin E was transformed to prostaglandin B, and 19-OH prostaglandin E to 19-OH prostaglandin B. Prostaglandins were separated by using Lipidex column chromatography and silicic acid column chromatography. Prostaglandin B was quantitated by gas chromatography using C24 fatty acid methyl ester as internal standard. 19-OH prostaglandin B was quantitated by measuring ultraviolet photoabsorbance at 278 nm.