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Related Experiment Videos

Are lymphocyte subset determinations affected by storage conditions?

A D Ponzio, S E Self, D H Runey

    Diagnostic Immunology
    |January 1, 1984
    PubMed
    Summary
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    Storing peripheral whole blood samples overnight at room temperature or 4°C does not affect T cell subsets or HLA-DR-positive cells. Analysis within 24 hours confirms reliable cell counts regardless of short-term storage conditions.

    Area of Science:

    • Immunology
    • Cell Biology
    • Clinical Laboratory Science

    Background:

    • Accurate enumeration of immune cell subsets is crucial for clinical diagnostics and research.
    • Understanding the stability of peripheral blood cells under various storage conditions is essential for reliable sample handling.
    • Flow cytometry is a standard method for quantifying immune cell populations.

    Purpose of the Study:

    • To evaluate the impact of overnight storage at room temperature and 4°C on key immune cell populations.
    • To determine the stability of total T cells, helper T cells, suppressor T cells, and HLA-DR-positive cells in whole blood.
    • To assess the reliability of flow cytometry analysis for these cell subsets within a 24-hour timeframe.

    Main Methods:

    • Peripheral whole blood samples were stored overnight under two conditions: room temperature and 4°C.

    Related Experiment Videos

  • Cell populations including total T cells, helper cells, suppressor cells, and HLA-DR-positive cells were analyzed.
  • Flow cytometry utilizing fluoresceinated monoclonal antibodies was employed for cell enumeration.

    • Main Results:

    • No significant differences were observed in the counts of total T cells, helper cells, suppressor cells, or HLA-DR-positive cells.
    • Cell subset stability was maintained irrespective of whether samples were stored at room temperature or refrigerated at 4°C.
    • These findings held true when samples were analyzed within 24 hours of collection.

    Conclusions:

    • Overnight storage of peripheral whole blood at either room temperature or 4°C does not compromise the integrity of major T cell subsets or HLA-DR-positive cells.
    • Flow cytometry analysis of these immune cell populations is reliable when performed within 24 hours, regardless of short-term storage conditions.
    • This study supports the flexibility in sample handling protocols for immunological assays, provided analysis is timely.