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H-2-linked murine factor B phenotypes.

E S Paolucci, D C Shreffler

    Immunogenetics
    |January 1, 1983
    PubMed
    Summary
    This summary is machine-generated.

    A new assay reveals genetic control of Factor B (B) activity in mice. Differences in B activity levels are linked to the H-2 complex, specifically the S region, indicating structural variations in B molecules.

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    Area of Science:

    • Immunogenetics
    • Complement System
    • Mouse Genetics

    Background:

    • Factor B (B) is a crucial component of the complement system.
    • Genetic variations in complement proteins can influence immune responses.
    • Understanding the genetic basis of Factor B activity is important for immunology.

    Purpose of the Study:

    • To develop a specific hemolytic assay for murine Factor B (B) activity.
    • To investigate the genetic control of Factor B activity in different mouse strains.
    • To map the gene responsible for Factor B activity differences.

    Main Methods:

    • Developed a specific hemolytic assay for Factor B (B) activity in murine EDTA-plasma.
    • Analyzed Factor B activity levels in various B10-congenic mouse strains and interspecific crosses.

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  • Utilized intra-H-2 recombinants for gene mapping.
  • Performed radial immunodiffusion to assess antigenic levels.
  • Conducted mixing experiments to rule out inhibitors or activators.

    • Main Results:

    • Identified three discrete levels of Factor B activity among B10-congenic strains.
    • Observed significant differences in Factor B activity associated with specific H-2 haplotypes (e.g., H-2v, H-2ja, H-2wr7).
    • Mapped the gene controlling Factor B activity to the S region of the H-2 complex, closely linked to the Ss locus.
    • Demonstrated intermediate activity in F1 generations, suggesting codominant inheritance.
    • Found no differences in antigenic protein levels, indicating functional rather than quantitative variations.

    Conclusions:

    • Factor B activity is controlled by a single gene located in the H-2-linked S region.
    • Allelic variations in this gene lead to structural differences in Factor B molecules, affecting their specific hemolytic activity.
    • These findings provide insights into the genetic regulation of the complement system in mice.