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Related Experiment Videos

Deformability characteristics of sickle cells by microelastimetry.

T C Havell, D Hillman, L S Lessin

    American Journal of Hematology
    |January 1, 1978
    PubMed
    Summary

    Sickle cell erythrocytes show altered deformability compared to normal cells. Irreversibly sickled cells exhibit increased membrane rigidity, contributing to the pathology of sickle cell disease.

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    Area of Science:

    • Hematology
    • Biophysics
    • Cell Biology

    Background:

    • Sickle cell disease is characterized by abnormal red blood cell shape and function.
    • Erythrocyte deformability is crucial for blood flow and oxygen delivery.
    • Alterations in red blood cell membrane properties are implicated in sickle cell disease pathology.

    Purpose of the Study:

    • To quantify the deformability and intrinsic membrane rigidity of normal and sickle erythrocytes.
    • To investigate the changes in membrane properties associated with the sickling-unsickling process.
    • To correlate membrane abnormalities with the pathophysiology of sickle cell disease.

    Main Methods:

    • Micropipette aspiration elastimetry was used to measure negative pressure.
    • Intrinsic membrane rigidity (P) and total cell deformability (Pt) were determined.
    • Measurements were conducted on oxygenated sickle discocytes, normal discocytes, and irreversibly sickled cells (ISCs).

    Main Results:

    • Oxygenated sickle discocytes showed similar membrane rigidity but higher total deformability than normal discocytes.
    • Irreversibly sickled cells (ISCs) exhibited significantly increased membrane rigidity and whole cell deformability compared to normal cells.
    • ISCs demonstrated elastic deformation, unlike the elastic reversion seen in normal and sickle discocytes.

    Conclusions:

    • The sickling-unsickling process progressively alters the sickle cell membrane.
    • Irreversibly sickled cells possess abnormal membrane rigidity and deformability.
    • ISC membrane abnormalities likely play a role in the pathological rheology of sickle cell disease.

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