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Related Experiment Videos

Virus-like particles in winter vomiting disease.

H Appleton, M Buckley, B T Thom

    Lancet (London, England)
    |February 19, 1977
    PubMed
    Summary

    A primary school outbreak of winter vomiting disease revealed novel virus-like particles in patient stool samples. These particles were not cultivable and antigenically distinct from known agents, suggesting a new cause of viral gastroenteritis.

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    Area of Science:

    • Gastroenterology
    • Virology
    • Epidemiology

    Background:

    • Winter vomiting disease outbreaks are common in school settings.
    • Identifying the causative agent is crucial for control and prevention.

    Purpose of the Study:

    • To characterize the virus-like particles associated with a primary school outbreak of winter vomiting disease.
    • To determine if the identified particles are antigenically related to known gastroenteritis viruses.

    Main Methods:

    • Electron microscopy was used to examine faecal specimens.
    • Buoyant density in caesium chloride was determined.
    • Immune electron microscopy was performed to assess antigenic properties.
    • Tissue-culture and organ-culture were attempted for virus isolation.

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    Main Results:

    • Virus-like particles (26 nm diameter) were detected in 7/8 faecal samples.
    • Particles had a buoyant density of 1.38–1.40 g/cm³.
    • The particles were not culturable using standard methods.
    • Antigenic analysis indicated differences from Norwalk and Hawaii agents.
    • Viral shedding persisted for over one month in some patients.

    Conclusions:

    • The study identified a distinct virus-like particle associated with a winter vomiting disease outbreak.
    • The findings suggest a potentially novel viral agent responsible for gastroenteritis in this outbreak.
    • Further characterization is needed to confirm the agent's identity and role.