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[Clinical evaluation of kininogenesis indices]
Insights
Plasma kallikrein activity discrepancies were observed in vitro and in children with bronchopulmonary diseases. Kininogenase activity measurement is the objective method for assessing plasma kallikrein function.
Area of Science:
- Biochemistry
- Clinical Chemistry
- Pediatrics
Context:
- Plasma kallikrein plays a crucial role in the kallikrein-kinin system.
- Assessing plasma kallikrein activity is important for understanding various physiological and pathological processes.
- Bronchopulmonary diseases in young children can be associated with alterations in the kallikrein-kinin system.
Purpose:
- To investigate discrepancies in plasma kallikrein activity measurements using different assays.
- To evaluate the reliability of various methods for determining plasma kallikrein, prekallikrein, and kallikrein inhibitor levels.
- To establish the most objective method for assessing kininogenesis intensity in clinical settings.
Summary:
- Experiments in vitro and studies on children under 3 with bronchopulmonary diseases revealed discrepancies between kininogenase, BAEE-esterase, and BAPNA-amidase activities of plasma kallikrein.
- The esterase technique broadly measures contact activation factors, not specific kallikrein activity.
- The test-tube chromatographic method is objective for kininogenase activity in fractions containing kallikrein or prekallikrein.
Impact:
- Identifies the limitations of esterase and chromatographic methods in accurately reflecting plasma kallikrein activity.
- Highlights kininogenase activity measurement as the most objective approach for assessing plasma kallikrein function.
- Provides crucial insights for accurate diagnostic assessments in pediatric respiratory diseases related to the kallikrein-kinin system.
Abstract:
The discrepancies between kininogenase, BAEE-esterase and BAPNA-amidase activity in plasma kallikrein were shown in experiments in vitro. The same discrepancies were detected during examination of children aged under 3 years suffering from bronchopulmonary diseases (acute and lingering pneumonia, bronchial asthma). In these children, prekallikrein, kallikrein and kallikrein inhibitor were determined at a time by the 3 methods (esterase, test-tube chromatographic and kininogenase ones). It has been shown that the kininogenesis intensity could be assessed objectively only in the measurement of the kininogenase activity of kallikrein contained by the whole plasma or its fractions. The esterase technique characterizes total factors of contact activation and does not reflect the activity of kallikrein, prekallikrein and kallikrein inhibitor. The test-tube chromatographic method might be regarded as objective only in the measurement of the kininogenase activity of the fractions that contain kallikrein or prekallikrein.