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Structural aspects of human kininogens.

W Muller-Esterl, B Dittmann, H Fritz

    Advances in Experimental Medicine and Biology
    |January 1, 1983
    PubMed
    Summary
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    Human kininogens, proteins found in plasma, exist in low molecular weight (LMW) and high molecular weight (HMW) forms. These kininogens can self-aggregate, a process potentially mediated by their heavy chains.

    Area of Science:

    • Biochemistry
    • Proteomics
    • Molecular Biology

    Background:

    • Kininogens are precursors to kinins, vasoactive peptides involved in various physiological processes.
    • Human plasma contains two main kininogen forms: low molecular weight (LMW) and high molecular weight (HMW).

    Purpose of the Study:

    • To purify and characterize human LMW and HMW kininogens.
    • To investigate the self-aggregation properties of purified kininogens.

    Main Methods:

    • Purification of kininogens from human plasma to homogeneity.
    • Limited proteolysis using tissue kallikrein to analyze protein structure.
    • Molecular weight determination using SDS-PAGE.
    • Sequence analysis of kininogen light chains.
    • Investigation of self-association using dissociating agents.

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    Main Results:

    • Native human LMW kininogen (68,000 MW) and HMW kininogen (114,000 MW) were purified.
    • Proteolysis by tissue kallikrein converted LMW kininogen into heavy (62,000 MW) and light (4,000 MW) chains, and HMW kininogen into heavy (58,000 MW) and light (62,000 MW) chains.
    • Sequence analysis revealed partial homology between human LMW kininogen light chain and bovine HMW kininogen fragment-1.2.
    • Purified kininogens formed reversible self-aggregates, from dimers to decamers (LMW) and hexamers (HMW).
    • Preliminary data suggest the heavy chain mediates kininogen self-association.

    Conclusions:

    • Human LMW and HMW kininogens exhibit distinct molecular weights and proteolytic cleavage patterns.
    • Kininogens possess self-aggregating properties, reversible by dissociating agents.
    • The heavy chain of kininogens appears to play a role in their oligomerization.