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Studies in sarcoidosis: intradermal Mycoplasma test
The American Review of Respiratory Disease
|May 1, 1978
Summary
A novel Mycoplasma test, derived from human macrophages, showed significantly higher positive reactions in sarcoidosis patients compared to other disease groups. This suggests a potential diagnostic marker for sarcoidosis.
Area of Science:
- Immunology
- Microbiology
- Infectious Diseases
Background:
- Mycoplasma orale is a species of mollicutes.
- Mycoplasma strain 215-M is closely related to Mycoplasma orale type 1.
- Human macrophages play a role in immune responses.
Purpose of the Study:
- To investigate the potential of Mycoplasma strain 215-M as a diagnostic tool for sarcoidosis.
- To evaluate skin test reactivity in patients with sarcoidosis and other diseases.
Main Methods:
- Mycoplasma strain 215-M was cultured in human macrophage monolayers in vitro.
- Sonicated macrophage homogenates were prepared and diluted in saline.
- Intradermal skin tests were performed on 19 sarcoidosis patients and 25 patients with other diseases.
Main Results:
- Positive skin-test reactions were observed significantly more frequently in patients with sarcoidosis.
- The Mycoplasma test material showed differential reactivity between sarcoidosis patients and controls.
Conclusions:
- Mycoplasma strain 215-M antigen may serve as a useful diagnostic indicator for sarcoidosis.
- Further research is warranted to validate this Mycoplasma-based skin test for sarcoidosis diagnosis.