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Related Experiment Videos

Standardization of serum protein analyses.

B Klein

    Annals of Clinical and Laboratory Science
    |May 1, 1978
    PubMed
    Summary

    Bovine serum albumin is a crucial standard for measuring total protein in serum. It is now available as a reference material, alongside updated immunoglobulin standards.

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    Area of Science:

    • Biochemistry
    • Clinical Chemistry
    • Analytical Chemistry

    Background:

    • The accurate determination of serum total protein is essential in clinical diagnostics.
    • Historically, various proteins have been evaluated for use as standards.
    • The need for reliable and standardized protein measurements has driven the development of reference materials.

    Purpose of the Study:

    • To provide a historical overview of bovine serum albumin's development as a standard for serum total protein determination.
    • To update on the availability of bovine serum albumin as a standard reference material.
    • To review the current status of immunoglobulin standards.

    Main Methods:

    • Historical literature review.
    • Examination of the availability of bovine serum albumin as a certified reference material.
    • Review of current literature and standards for immunoglobulins.

    Main Results:

    • Bovine serum albumin has evolved as a widely accepted standard for serum total protein assays.
    • The National Bureau of Standards now provides bovine serum albumin as a standard reference material.
    • Current immunoglobulin standards have also been assessed and reviewed.

    Conclusions:

    • Bovine serum albumin serves as a critical reference standard in clinical protein analysis.
    • The availability of standardized reference materials enhances the reliability and comparability of laboratory results.
    • Ongoing evaluation of immunoglobulin standards ensures their continued utility in diagnostics.

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