Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Strain differentiation in methicillin-resistant Staphylococcus aureus.

A M Vickery, M A Beard-Pegler, P M Rountree

    Pathology
    |July 1, 1983
    PubMed
    Summary
    This summary is machine-generated.

    Related Concept Videos

    You might also read

    Related Articles

    Articles linked to this work by shared authors, journal, and citation graph.

    Sort by
    Same author

    Hospital blankets as a source of infection.

    The Medical journal of Australia·2010
    Same author

    The treatment of hospital blankets with oil emulsions and the bactericidal action of fixanol C (cetyl pyridinium bromide).

    The Medical journal of Australia·2010
    Same author

    A comparison of the tensile strengths of iodine sterilized and heat sterilized catgut sutures.

    The Australian and New Zealand journal of surgery·2010
    Same author

    Staphylococcal bacteriophages; the effect of penicillin on staphylococcal bacteriophages.

    The Australian journal of experimental biology and medical science·2010
    Same author

    Cross-infection of wounds in a surgical ward during a trial of the use of oiled blankets.

    The Medical journal of Australia·2010
    Same author

    An epidemiological survey of methicillin-resistant Staphylococcus aureus in a tertiary referral hospital.

    The Journal of hospital infection·2000
    Same journal

    Impact of the 2025 Endocrine Society clinical practice guideline on the diagnosis and management of primary aldosteronism for the clinical laboratory.

    Pathology·2026
    Same journal

    Cribriform intraductal carcinoma of the prostate may have a greater prognostic impact even than Gleason grade 5 conventional prostatic adenocarcinoma.

    Pathology·2026
    Same journal

    Bordetella pertussis: emerging epidemiology, diagnosis and management.

    Pathology·2026
    Same journal

    Development and validation of a real-time polymerase chain reaction for the detection of Haycocknema perplexum.

    Pathology·2026
    Same journal

    Recent advances in the understanding of TP53 in haematological malignancies.

    Pathology·2026
    Same journal

    Concurrent renal amyloid light chain amyloidosis, light chain crystalline podocytopathy and light chain proximal tubulopathy: a case report.

    Pathology·2026
    See all related articles

    Methicillin-resistant Staphylococcus aureus (MRSA) strains causing hospital cross-infections in NSW were investigated. Phage typing revealed multiple strains, with a predominant type and several non-typable isolates, necessitating new phages for clearer identification.

    Area of Science:

    • Microbiology
    • Infectious Diseases
    • Epidemiology

    Background:

    • Methicillin-resistant Staphylococcus aureus (MRSA) is a significant cause of hospital-acquired infections.
    • Cross-infection by MRSA poses a substantial challenge in healthcare settings, particularly in New South Wales (NSW).
    • Understanding the strain diversity of MRSA is crucial for effective infection control.

    Purpose of the Study:

    • To determine the strain diversity of MRSA isolates responsible for cross-infections in NSW hospitals.
    • To evaluate the utility of biochemical tests and phage typing in differentiating MRSA strains.
    • To identify novel phage typing methods for improved MRSA strain characterization.

    Main Methods:

    • Analysis of 236 MRSA isolates from NSW hospitals.

    Related Experiment Videos

  • Application of biochemical testing methods.
  • Phage typing using the Basic International Set of phages at 100x routine test dilution (RTD).
  • Isolation and characterization of five new phages for improved typing.

    • Main Results:

    • Biochemical tests provided limited discriminatory power for MRSA strains.
    • Phage typing indicated the presence of multiple MRSA strains, with a predominant type (83A/85/95/90/88).
    • A significant proportion of isolates were not typable with the standard phage set, and results were often difficult to interpret.
    • Newly isolated phages yielded easily identifiable patterns at RTD, enhancing typing accuracy.

    Conclusions:

    • Multiple MRSA strains contribute to hospital cross-infections in NSW.
    • Standard phage typing methods have limitations in characterizing MRSA diversity.
    • The development of new phages significantly improves the ability to differentiate and track MRSA strains, aiding infection control efforts.