Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Plant-animal cell fusions.

E C Cocking

    Ciba Foundation Symposium
    |January 1, 1984
    PubMed
    Summary

    High pH and calcium methods enhance plant and animal cell fusion compared to older techniques. Further research is needed to confirm gene function in these interkingdom heterokaryons.

    Related Concept Videos

    You might also read

    Related Articles

    Articles linked to this work by shared authors, journal, and citation graph.

    Sort by
    Same author

    An electron microscopic study of the initial stages of infection of isolated tomato fruit protoplasts by tobacco mosaic virus.

    Planta·2014
    Same author

    Protein synthesis in tomato fruit locule tissue: The sites of synthesis and the pathway of carbon into protein.

    Planta·2014
    Same author

    Isolation of protoplasts from cereal leaves.

    Planta·2014
    Same author

    Somatic hybrids between unilateral cross-incompatible Petunia species.

    TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik·2013
    Same author

    Somatic hybridization of sexually incompatible petunias: Petunia parodii, Petunia parviflora.

    TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik·2013
    Same author

    Restriction endonuclease analysis of chloroplast DNA in interspecies somatic Hybrids of Petunia.

    TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik·2013

    Area of Science:

    • Cell biology
    • Biotechnology
    • Interkingdom fusion

    Background:

    • Plant protoplast and animal cell fusion is crucial for research.
    • Previous methods like polyethylene glycol (PEG) have limitations.

    Purpose of the Study:

    • To compare different methods for plant-animal cell fusion.
    • To investigate factors influencing interkingdom fusion success.
    • To analyze the survival and genetic function of resulting heterokaryons.

    Main Methods:

    • Comparing high pH/high Ca2+ method with PEG-induced fusion.
    • Utilizing immunofluorescence to study plasma membrane interactions.
    • Investigating culture medium effects on heterokaryon survival and dominance.

    Main Results:

    • High pH/high Ca2+ method demonstrated faster and more complete cytoplasmic fusion than PEG.
    • Immunofluorescence revealed plasma membrane interactions in plant-animal heterokaryons.
    • Culture medium significantly impacts heterokaryon survival and cell partner dominance.

    Conclusions:

    • High pH/high Ca2+ method is a promising technique for interkingdom fusion.
    • Further investigation is required to determine if both plant and animal genotypes are functional in heterokaryons.

    Related Experiment Videos