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R factors from Enterobacter group
Abstract:
Twenty-six R factors transmissible to E. coli K12 were derived from 79 strains of Enterobacter species isolated in various clinical specimens (urine, sputum, blood, pus); 71 of 79 strains were Enterobacter cloacae. Fifteen R factors were fi+: 14 belonged to group FII, 1 to group FI. Eleven R factors were fi-: 5 belonged to group M, of the others 6 (all controlling the cloramphenicol-resistance) 1 was recognized of group K, 1 was incompatible with standard plasmids of both groups S and W, and 4 belonged to group S.
Insights
Researchers identified 26 antibiotic resistance (R) factors in Enterobacter species, primarily Enterobacter cloacae, from clinical samples. These R factors, transmissible to E. coli K12, were characterized by their incompatibility groups, revealing insights into bacterial resistance mechanisms.
Area of Science:
- Microbiology
- Molecular Biology
- Genetics
Background:
- Antibiotic resistance is a growing public health concern.
- Enterobacter species are common causes of hospital-acquired infections.
- R factors (resistance plasmids) contribute to the spread of antibiotic resistance.
Purpose of the Study:
- To characterize R factors found in clinical isolates of Enterobacter species.
- To determine the transmissibility and incompatibility groups of these R factors.
Main Methods:
- Isolation of Enterobacter species from clinical specimens.
- Derivation of R factors transmissible to Escherichia coli K12.
- Classification of R factors based on their incompatibility groups.
Main Results:
- Twenty-six R factors were successfully derived from 79 Enterobacter strains.
- The majority of isolates (71/79) were identified as Enterobacter cloacae.
- Fifteen R factors were fertility-positive (fi+), with 14 belonging to group FII and 1 to group FI.
- Eleven R factors were fertility-negative (fi-), belonging to groups M, K, S, or showing incompatibility with S and W groups.
Conclusions:
- Enterobacter cloacae is a significant reservoir for transmissible R factors.
- The characterized R factors represent diverse incompatibility groups, indicating varied plasmid origins and potential for spread.
- Understanding R factor diversity is crucial for combating the spread of antibiotic resistance in clinical settings.