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Immunoreactive tissue kallikrein in human serum.
The Journal of Laboratory and Clinical Medicine
|May 1, 1984
Summary
This study developed a radioimmunoassay to measure human tissue kallikrein in serum. Elevated levels were observed in pancreatitis and with low sodium diets, suggesting a role in vascular resistance.
Area of Science:
- Biochemistry
- Physiology
- Immunology
Background:
- Human urinary kallikrein plays a role in physiological processes.
- Quantifying tissue kallikrein in serum is crucial for understanding its function.
- Existing methods may not accurately detect serum kallikrein.
Purpose of the Study:
- To develop and validate a radioimmunoassay for human tissue kallikrein in serum.
- To investigate factors influencing serum tissue kallikrein levels.
- To assess the potential of serum kallikrein as a biomarker.
Main Methods:
- Radioimmunoassay using 125I-labeled human urinary kallikrein and rabbit antiserum.
- Assay validation with serum, saliva, and pancreatic extracts.
- Measurement of serum kallikrein in normal volunteers and patient groups.
Main Results:
- The radioimmunoassay demonstrated high sensitivity (160 pg/ml) and specificity.
- Serum kallikrein levels were significantly elevated in acute pancreatitis and with low sodium intake.
- Levels were unchanged in Fletcher trait and Hageman factor deficiency, and unaffected by plasma kallikrein or prekallikrein.
Conclusions:
- A reliable method for quantifying immunoreactive tissue kallikrein in human serum has been established.
- Serum tissue kallikrein levels are influenced by dietary sodium and pancreatitis.
- This assay provides a new tool for studying the kallikrein-kinin system and its role in vascular resistance regulation.