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Related Experiment Videos

Detergent-resistant Streptococcus faecium derivatives that display conditional penicillin lysis.

M J Pucci, L Daneo-Moore

    Journal of Bacteriology
    |August 1, 1984
    PubMed
    Summary

    Certain Streptococcus faecium strains show reduced lysis and killing at 25 degrees C, but adding unsaturated fatty acids restores normal penicillin-induced lysis rates. This highlights temperature-dependent cell wall integrity influenced by fatty acid composition.

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    Area of Science:

    • Microbiology
    • Cell Biology
    • Biochemistry

    Background:

    • Streptococcus faecium ATCC 9790 is a bacterium known for its cell wall properties.
    • Temperature can significantly impact bacterial physiology and cell wall integrity.
    • Triton X-100 resistance can be an indicator of altered membrane composition.

    Purpose of the Study:

    • To investigate the lysis rates of Streptococcus faecium derivatives under different temperature conditions.
    • To determine the effect of exogenous unsaturated fatty acids on penicillin-induced lysis at low temperatures.
    • To understand the role of membrane fatty acid composition in bacterial cell wall stability.

    Main Methods:

    • Isolation of spontaneous Streptococcus faecium derivatives.
    • Assessment of penicillin-induced lysis rates at 25 degrees C and 37 degrees C.

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  • Supplementation with exogenous unsaturated fatty acids at 25 degrees C.

    • Main Results:

    • Spontaneous derivatives exhibited normal lysis at 37 degrees C but reduced lysis and killing at 25 degrees C.
    • Addition of unsaturated fatty acids at 25 degrees C restored wild-type penicillin lysis rates.
    • These findings suggest temperature-dependent alterations in cell wall function.

    Conclusions:

    • Bacterial lysis and killing by penicillin are temperature-sensitive in certain Streptococcus faecium strains.
    • Membrane fatty acid composition plays a crucial role in maintaining cell wall integrity and susceptibility to lysis at lower temperatures.
    • Exogenous unsaturated fatty acids can rescue temperature-induced defects in cell wall function.