[Electrophoretic and chromatographic studies of zein]

Total zein is isolated from fat-free maize flour and studied using electrophoresis in acrylamide gel gradient (7-30%) and gel filtration through Sephacryl S-200 (Superfine) and Sepharose CL-6B. Molecular weights of electrophoretic and chromatographic fractions are determined. 12 electrophoretic and 5-7 chromatographic fractions are found in zein, their molecular weight being 40000-280000 daltons.