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Related Experiment Videos

Renotrophic factors in urine.

R H Harris, M K Hise, C F Best

    Kidney International
    |April 1, 1983
    PubMed
    Summary

    Urine contains renotrophic factors that stimulate kidney growth. These factors, retained in urine through a "half-urine-reinfusion" model, promote renal DNA and phospholipid synthesis, indicating a direct action on kidney tissue.

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    Area of Science:

    • Nephrology
    • Renal Physiology
    • Molecular Biology

    Background:

    • Previous observations suggested circulatory renotrophic factors in urine stimulate renal growth.
    • A model of reinfusing half of an animal's urine output was used to study renal excretory function.

    Purpose of the Study:

    • To investigate the role of urinary renotrophic factors in stimulating renal growth.
    • To characterize the nature and origin of these renotrophic factors.

    Main Methods:

    • Utilized a "half-urine-reinfusion" rat model to selectively reduce renal excretory function.
    • Developed a bioassay involving intravenous infusion of urine into assay rats.
    • Employed ultrafiltration and heat treatment to isolate and characterize renotrophic activity.
    • Assessed DNA and phospholipid synthesis in renal and hepatic tissues.

    Main Results:

    • Demonstrated significant increases in renal DNA content and thymidine incorporation in the "half-urine-reinfusion" model.
    • The urine bioassay showed increased renal DNA and phospholipid synthesis.
    • Renotrophic activity was heat-stable and associated with molecules >10,000 daltons.
    • Urinary renotrophic factors appear to be of circulatory origin, not solely renal.
    • Isolated renal cortical fragments showed dose-dependent phospholipid synthesis stimulation.

    Conclusions:

    • Urinary renotrophic factors are capable of stimulating renal growth and synthesis.
    • These factors likely originate from the circulation and act directly on kidney tissue.
    • The findings support the hypothesis of circulatory retention of factors promoting renal hypertrophy.

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