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Folate utilization in Friend erythroleukemia cells.

S E Steinberg, S Fonda, C L Campbell

    Journal of Cellular Physiology
    |February 1, 1983
    PubMed
    Summary
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    Friend erythroleukemia cells maintain intracellular folate pools proportional to pteroylglutamic acid (PteGlu1) levels. The size of the polyglutamyl folate pool dictates replication ability, while cell doubling time controls folate turnover.

    Area of Science:

    • Cell Biology
    • Biochemistry
    • Nutritional Science

    Background:

    • Endogenous folate pools are crucial for cellular functions.
    • Understanding folate metabolism is key to preventing deficiencies.

    Purpose of the Study:

    • To investigate folate pool generation and regulation in Friend erythroleukemia cells.
    • To determine the functional importance of intracellular folate pools for cell replication.

    Main Methods:

    • Friend erythroleukemia cells were cultured with varying concentrations of tritiated pteroylglutamic acid (3H)PteGlu1.
    • Intracellular folate pools were analyzed for polyglutamyl folate content.
    • Cell replication rates were measured under different folate conditions.

    Main Results:

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    • Intracellular folate levels directly correlated with exogenous pteroylglutamic acid (PteGlu1) concentration.
    • Over 95% of intracellular folate existed as polyglutamyl folate at equilibrium.
    • Replication capacity was dependent on the intracellular polyglutamyl folate pool, with maximal rates requiring exogenous PteGlu1.

    Conclusions:

    • Cellular folate pools are directly influenced by external folate availability.
    • Intracellular polyglutamyl folate is essential for cell proliferation.
    • Folylpolyglutamate synthesis and dilution impact folate status in rapidly dividing cells.