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Serum-controlled membrane dynamic changes in growing human leukemia and lymphoma cells
Cancer Biochemistry Biophysics
|January 1, 1983
Summary
Cell membrane dynamics, measured by fluorescence polarization, show cyclic variations linked to cell growth. These changes are influenced by serum factors and vary between cell lines, impacting proliferation rates.
Area of Science:
- Cell Biology
- Biophysics
- Cancer Research
Background:
- Cell membrane dynamics are crucial for cellular functions, including growth and proliferation.
- Understanding membrane fluidity and its relationship with cell cycle progression is key in cancer research.
Purpose of the Study:
- To investigate the relationship between membrane dynamics and cell growth in various human leukemia-lymphoma cell lines.
- To identify factors influencing membrane fluidity variations during the cell cycle.
Main Methods:
- Utilized fluorescence polarization (P) with diphenylhexatriene probe to measure membrane dynamics.
- Studied B, T, and nonB-nonT phenotype leukemia-lymphoma cell lines.
- Manipulated cell culture medium (dialyzed vs. fresh serum) and serum concentration to cell number ratio.
Main Results:
- Observed cyclic variations in membrane polarization (P) correlating with the cell cycle, decreasing before logarithmic growth and increasing afterward.
- Membrane dynamic variations were independent of cell line phenotype or origin.
- Changes in P were dependent on fresh serum presence and the serum concentration to cell number ratio.
- Proliferation rates correlated with P modulations, except in immature non B-non T REH cells.
Conclusions:
- Cell membrane component organization dynamics are cell-specific.
- Serum factors play a critical role in controlling these membrane dynamic changes.
- Membrane dynamics are linked to cell proliferation, with some exceptions in immature cell lines.