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Lipids of supragingival calculus
Journal of Dental Research
|August 1, 1983
Summary
The study reveals supragingival calculus contains significant protein and lipids, including neutral lipids, glycolipids, and phospholipids. Both salivary and bacterial sources contribute to the calculus lipid profile.
Area of Science:
- Biochemistry
- Oral Biology
- Dental Research
Background:
- Supragingival calculus is a complex dental biofilm.
- Understanding its composition is crucial for oral health research.
Purpose of the Study:
- To analyze the lipid and protein composition of supragingival calculus.
- To investigate the origins of lipids within calculus.
Main Methods:
- Gravimetric analysis of calculus dry weight.
- Lipid extraction and fractionation.
- Identification and quantification of lipid classes and subclasses.
Main Results:
- Calculus matrix is 15.7% of dry weight, with 54.9% protein and 10.2% lipids.
- Lipids comprise neutral lipids (61.8%), glycolipids (28%), and phospholipids (10.2%).
- Neutral lipids are rich in free fatty acids; phospholipids include phosphatidylethanolamine.
Conclusions:
- Supragingival calculus has a distinct lipid profile.
- Both salivary components and bacterial metabolism contribute to calculus lipids.