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A simple colorimetric method for ascorbic acid determination in blood plasma.

A Kyaw

    Clinica Chimica Acta; International Journal of Clinical Chemistry
    |June 1, 1978
    PubMed
    Summary
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    A new, simple method accurately determines ascorbic acid (vitamin C) in plasma. Acid phosphotungstate offers a specific and reproducible reagent for vitamin C analysis.

    Area of Science:

    • Biochemistry
    • Analytical Chemistry
    • Clinical Chemistry

    Background:

    • Ascorbic acid (vitamin C) is a vital nutrient and antioxidant.
    • Accurate determination of ascorbic acid in biological samples like plasma is crucial for health assessment.
    • Existing methods may have limitations in specificity, sensitivity, or reproducibility.

    Purpose of the Study:

    • To develop and validate a simple, specific, and sensitive method for ascorbic acid determination.
    • To assess the applicability of the method for routine analysis in human plasma.
    • To evaluate the reproducibility of the proposed method.

    Main Methods:

    • Utilized acid phosphotungstate as a reagent for ascorbic acid detection.
    • Applied the method to plasma samples for ascorbic acid quantification.

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  • Performed reproducibility tests to ensure reliability.

    • Main Results:

    • The acid phosphotungstate reagent demonstrated high specificity for ascorbic acid.
    • The method proved to be sensitive, enabling accurate quantification.
    • Good reproducibility was achieved when determining ascorbic acid in plasma samples.

    Conclusions:

    • The described method provides a simple, specific, and sensitive approach for ascorbic acid determination.
    • Acid phosphotungstate is a suitable reagent for reliable ascorbic acid analysis in plasma.
    • This method holds potential for routine clinical and research applications.