Adhesives for retaining prefixed urothelial cells on slides after imprinting from cellulosic filters

Cells of prefixed urine specimens collected on Millipore filters can be imprinted onto labelled microslides coated with adhesives. The proportion of cells transferred to the slides and recovered from the filters was investigated using three different adhesives. Egg albumen-glycerin and Apathy's syrup did not increase the transfer of cells as compared with experiments without adhesives; the proportion of transferred cells varied from less than 10% to more than 70%. Gelatin-chrome alum had, with minor variations, a pronounced adhesive effect: at least 94% of the cells was always recovered after the cells were transferred from filters to slides. Practical details, including advantages of the filter imprint method, are discussed.