Quail fetal antigen (QFA): identification and comparison with chicken fetal antigen

Quail fetal antigen (QFA), an analogous hematopoietic antigen to chicken fetal antigen (CFA), was identified and shown to be a developmental antigen on the blood cells of Japanese quail and other avian species. Rabbit antiserum against 14-day embryonic quail red blood cells (RBCs) was specifically adsorbed to achieve fetal specificity and to eliminate any cross reactivity with the CFA system. Complement-mediated microcytotoxicity and hemagglutination assays were used to detect the presence of QFA on hematopoietic cells. QFA was found on 100% of the peripheral RBCs from 10-day quail embryos but incidence of the antigen gradually declined with increasing development. Complete loss of QFA from RBCs occurred just prior to sexual maturation between 31 and 42 days of age. No qualitative differences in erythroid expression of QFA were observed during development; however, RBCs from both embryonic duck and interspecific quail- chicken hybrids reacted with R-anti QFA. Like CFA, quail fetal antigen was associated with lymphocytes, particularly those from primary lymphoid organs. Similarities in the developmentally controlled tissue distribution of QFA and CFA suggest that developmental hematopoietic antigens are a common feature of avian species and are useful cell surface markers for studies of development and differentiation.