Related Experiment Video
Updated: Aug 17, 2026

Isolating Primary Melanocyte-like Cells from the Mouse Heart
Published on: September 29, 2014
Ionic regulation of MEL cell commitment
Abstract:
A key event in the initiation of the dimethyl sulfoxide (DMSO)-induced program of murine erythroleukemia (MEL) cell differentiation is a rise in the level of cytoplasmic calcium ions. Our interest in the present study is whether other inducers of the terminal erythroid differentiation program also act via a calcium-dependent pathway. Inhibition of calcium transport has been found to prevent the induction of MEL cell commitment by DMSO, butyric acid (BA), or hypoxanthine (HX). Enhancement of the calcium flux rate with A23187 or elevation of cytoplasmic calcium levels with FCCP stimulates the kinetics of commitment in response to all three inducers. These results suggest that of the inducers we have tested (DMSO, BA, and HX), all three act to initiate commitment via a common mechanism which involves modulation of cytoplasmic calcium levels.
Related Concept Videos
Positive Regulator Molecules
Determination
Master Transcription Regulators
Lineage Commitment
Maintenance of the ES Cell State
Somatic to iPS Cell Reprogramming

