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Laser-induced multiphoton processes in living cells.

P P Calmettes, M W Berns

    Proceedings of the National Academy of Sciences of the United States of America
    |December 1, 1983
    PubMed
    Summary

    Specific light absorption causes subcellular changes, known as phase paling. This process requires multiple photons, with green light needing four and UV light needing two for visible effects in cell structures.

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    Area of Science:

    • Cell Biology
    • Photophysics
    • Biophysics

    Background:

    • Subcellular alterations, termed phase paling, are light-induced phenomena.
    • Understanding the underlying photophysical mechanisms is crucial for cell biology research.

    Purpose of the Study:

    • To investigate the multiphoton absorption processes responsible for light-induced phase paling.
    • To determine the photon requirements for phase paling in specific cellular components.

    Main Methods:

    • Exposure of cellular components (chromosomes, nucleoli) to specific wavelengths of light (green and UV).
    • Analysis of light-induced subcellular alterations (phase paling) to determine photon absorption order.

    Main Results:

    • Phase paling is confirmed to result from multiphoton absorption.
    • Inducing phase paling in chromosomes and nucleoli with green light (532 nm) requires a four-photon absorption process.
    • A two-photon absorption process is sufficient to induce phase paling with UV light (266 nm).

    Conclusions:

    • The number of photons required for light-induced phase paling varies with light wavelength.
    • Different cellular components exhibit distinct responses to multiphoton absorption, influencing the observed paling effects.

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