Further studies on the composition of the acquired enamel pellicle

In the present study pellicle material was collected from human teeth 2, 4 and 6 hr after cleaning. The material obtained was examined by gel filtration, ion exchange chromatography on CM-Sephadex and DEAE-Sephadex with subsequent amino acid analyses of the major anionic component. No major changes were observed to occur either in the overall composition or in the main anionic component of the pellicle during the first 6 hr.