Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Glycosylated ovine prolactin.

U J Lewis, R N Singh, L J Lewis

    Proceedings of the National Academy of Sciences of the United States of America
    |January 1, 1984
    PubMed
    Summary

    A newly discovered glycosylated prolactin from sheep pituitary glands shows altered biological activity. This modified hormone exhibits reduced potency and immunoreactivity compared to standard prolactin.

    Related Concept Videos

    You might also read

    Related Articles

    Articles linked to this work by shared authors, journal, and citation graph.

    Sort by
    Same author

    Computational Drug Design of Natural Product-Based Azole Hybrids for Multifactorial Diseases: Success Stories.

    Current topics in medicinal chemistry·2026
    Same author

    Associations of meteorological variability with sesame yield in semi-arid Bundelkhand region of India.

    Scientific reports·2026
    Same author

    Predicting yellow mosaic disease severity in yardlong bean using visible imaging coupled with machine learning model.

    Scientific reports·2025
    Same author

    Optimizing chickpea yield prediction under wilt disease through synergistic integration of biophysical and image parameters using machine learning models.

    Scientific reports·2025
    Same author

    Innovative and polygonal trend analysis of temperature in agro climatic zones of India.

    Scientific reports·2024
    Same author

    Rice yield prediction through integration of biophysical parameters with SAR and optical remote sensing data using machine learning models.

    Scientific reports·2024

    Area of Science:

    • Endocrinology
    • Glycobiology
    • Protein Chemistry

    Background:

    • Prolactin is a key pituitary hormone regulating numerous physiological processes.
    • Post-translational modifications, such as glycosylation, can significantly impact protein function.
    • The presence and role of glycosylated prolactin in vivo remain incompletely understood.

    Purpose of the Study:

    • To isolate and characterize a modified form of ovine prolactin.
    • To investigate the structural and functional consequences of prolactin glycosylation.
    • To compare the biological potency and immunoreactivity of glycosylated prolactin with native prolactin.

    Main Methods:

    • Isolation of glycosylated prolactin from ovine pituitary glands.
    • Sequence and amino acid analyses to determine carbohydrate linkage.
    • Lectins (concanavalin A, lentil lectin) used to confirm glycosylation.
    • Sodium dodecyl sulfate-gel electrophoresis (SDS-PAGE) for molecular weight determination.
    • Pigeon crop sac assay for biological potency.
    • Radioimmunoassay (RIA) for immunoreactivity assessment.

    Main Results:

    • A glycosylated prolactin variant was isolated, with carbohydrate attached at asparagine 31 via N-acetylglucosamine.
    • The modified prolactin exhibited a higher molecular weight (25,000 Da) compared to native prolactin (23,000 Da).
    • Glycosylated prolactin showed reduced biological potency (60% of reference) and significantly lower immunoreactivity (34%) in RIA.

    Conclusions:

    • Ovine prolactin can undergo glycosylation at Asn-31, resulting in a distinct molecular entity.
    • This glycosylation modification alters the hormone's interaction with antibodies and reduces its biological activity.
    • The findings provide insights into prolactin heterogeneity and its functional implications.

    Related Experiment Videos