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Presence of oncornavirus-like particles in the P388 murine leukemic cell line
Experientia
|May 15, 1978
Abstract:
A culture of P388 murine lymphoblastoid cells has been shown to contain type C oncornavirus-like particles budding at the plasma membrane. Occasionally intracytoplasmic type A and immature type B particles were also observed by electron microscope techniques. The discovery of oncornavirus-like particles in the P388 cell line increases the utility of this neoplastic system for detecting potential antineoplastic agents.
Insights
Murine lymphoblastoid cells (P388) contain oncornavirus-like particles. This finding enhances the P388 cell line
Area of Science:
- Oncology
- Virology
- Cell Biology
Background:
- The P388 murine lymphoblastoid cell line is a model system in cancer research.
- Oncornaviruses are a class of RNA viruses associated with cancer.
Purpose of the Study:
- To investigate the presence of viral particles within the P388 cell line.
- To assess the potential of the P388 cell line for antineoplastic drug screening.
Main Methods:
- Electron microscopy was used to examine P388 cell cultures.
- Observation of cellular structures and budding particles.
Main Results:
- Type C oncornavirus-like particles were observed budding from the plasma membrane of P388 cells.
- Intracytoplasmic type A and immature type B particles were occasionally detected.
- The presence of these viral particles was confirmed via electron microscopy.
Conclusions:
- The P388 cell line harbors oncornavirus-like particles.
- The identification of these particles enhances the utility of the P388 system for evaluating potential anticancer drugs.