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A sensitive and dependable assay for distinguishing hamster and human X-linked steroid sulfatase activity in somatic
Human Genetics
|January 1, 1984
Abstract:
A radioisotopic assay is described to distinguish between Chinese hamster and human steroid sulfatase activity in extracts prepared from hamster X human somatic cell hybrids. This assay is based on different pH optima and provides a sensitive and unambiguous biochemical marker for the short arm of the human X chromosome and as well as for otherwise genetically inactivated X chromosomes in rodent X human hybrids.