In vitro prostaglandin E2 stimulation of 45Ca mobilization from chick bone

The ability of prostaglandin E2 (PGE2) to mobilize 45Ca from chick embryo long bones was assayed in an in vitro bone culture system. Concentrations of PGE2 tested ranged from 10(-9) to 10(-5) M. The PGE2 was effective in stimulating release of 45Ca from prelabelled bones at all concentrations tested except at 10(-9) M. In addition, stimulation of 45Ca release could be produced daily for 4 consecutive days of PGE2 culture-pulsing at what appeared to be the optimal PGE2 concentration, 10(-7) M. We conclude, as in mammals, PGE2 is a potent stimulator of calcium mobilization from avian bone. The potential involvement of prostaglandins in eggshell formation is discussed.