Establishment of a cell line (TSUS-1) derived from a human squamous cell carcinoma of the penis

We established a cell line ( TSUS -1) derived from a relatively well-differentiated squamous cell carcinoma of the penis. The cells had been subcultured 60 times during two years and five months. The average doubling time of this cell line was 38 hours. Chromosomal analysis indicated that the modal chromosome number was 75; seven marker chromosomes and a male karyotype were present. The cultured cells were easily transplantable into BALB/c nude mice and the histological picture of the inoculated tumor was similar to that of the original tumor.