Serum and salivary IgG and IgA response to initial preparation therapy

The purpose of this study was to determine and compare IgG and IgA concentration in both serum and unstimulated whole saliva obtained prior to any therapy and following initial preparation therapy (IPT) in 15 healthy adult dentulous patients, five each of periodontal disease Classes I, II and III. During the first visit before therapy, 10 ml of whole blood and 5 ml of saliva were obtained. Serum protein electrophoresis was conducted to rule out immunoglobulinopathies. Radial cell immunodiffusion endpoint technique was employed on serum and saliva to determine IgG and IgA in mg/dl. Following IPT, achievement of plaque control and 3 weeks after any dental instrumentation, 10 ml of blood and 5 ml of saliva were obtained from each patient and IgG and IgA in mg/dl were determined. The following are trends that were observed: greater decrease in IgG and IgA in serum and saliva following IPT in Class I periodontal patients than in other classes; saliva was a better indicator of local humoral response than serum for this group of patients; the less severe the periodontal involvement, more consistent was the reduction in IgG and IgA following IPT, especially for saliva; IPT elicited a decreased IgG and IgA concentration when local antigenic stimuli were reduced by both patient and dentist.