Microradiographic and electron microscopic observations of the enamel-covered dentine in rat molars

The enamel-covered primary and secondary coronal dentine in the molars of 90-day-old and 360-day-old rats was examined using microradiography. Some preparations were subsequently examined by transmission and scanning electron microscopy (SEM). Fractured dentine surfaces and methacrylate casts of the tubular system in the primary dentine were also examined with SEM. No microradiographic evidence of a hypermineralized peritubular matrix, such as that seen in man and other species, was seen in either young or old rats. Transmission and SEM confirmed the microradiographic findings. The tubule obliteration and extensive intra-luminal mineral deposits which have been reported in the enamel-free coronal dentine of the rat molar were not seen in the enamel-covered coronal dentine but some evidence of tubule obliteration was seen in the secondary dentine.