Related Experiment Videos
Histologic effects of Amipaque (metrizamide) and various contrast media on mouse peritoneum
Abstract:
The histologic effects of Amipaque and various commonly used contrast media on mouse peritoneum were studied by intraperitoneal injections of Amipaque 370 mg I/ml and 170 mg I/ml, Conray 300 mg I/ml, Conray Meglumine 282 mg I/ml, Urografin 60% and 76%, Gastrografin and barium sulfate. Amipaque and the other water-soluble media cause no deleterious effects, whereas barium sulfate evokes granulomatous inflammatory reaction. Amipaque is suitable for gastrointestinal studies when leakage into the peritoneal cavity is expected.
Insights
Amipaque, a contrast agent, showed no adverse effects on the mouse peritoneum. Unlike barium sulfate, which caused inflammation, Amipaque is safe for gastrointestinal studies with potential leakage.
Area of Science:
- Medical imaging
- Histology
- Gastroenterology
Background:
- Contrast media are essential for medical imaging, but their effects on tissues require careful evaluation.
- Barium sulfate and various water-soluble contrast agents are commonly used.
Purpose of the Study:
- To compare the histologic effects of Amipaque with other contrast media on the mouse peritoneum.
- To determine the safety of Amipaque in gastrointestinal studies where peritoneal leakage is a concern.
Main Methods:
- Intraperitoneal injections of Amipaque (370 mg I/ml and 170 mg I/ml) were administered to mice.
- Other contrast media, including Conray, Urografin, Gastrografin, and barium sulfate, were also injected for comparison.
Main Results:
- Amipaque and other water-soluble contrast media did not cause significant deleterious effects on the peritoneum.
- Barium sulfate induced a granulomatous inflammatory reaction in the peritoneum.
Conclusions:
- Amipaque is histologically safe for the mouse peritoneum.
- Amipaque is a suitable contrast agent for gastrointestinal studies, even in cases of expected peritoneal leakage.