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Related Experiment Videos

7-Hydroxymethotrexate formation in a human lymphoblastic cell line.

P A Newton, R L Blakley

    Biochemical and Biophysical Research Communications
    |August 16, 1984
    PubMed
    Summary

    Methotrexate chemotherapy generates 7-hydroxymethotrexate, a less active metabolite. This conversion occurs within target cells, impacting acute lymphocytic leukemia treatment effectiveness.

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    Biochemical pharmacology·2000

    Area of Science:

    • Pharmacology
    • Oncology
    • Cell Biology

    Background:

    • Methotrexate is a key chemotherapy agent.
    • 7-Hydroxymethotrexate is a known metabolite of methotrexate.
    • Understanding metabolite formation is crucial for drug efficacy.

    Purpose of the Study:

    • To investigate the formation of 7-hydroxymethotrexate in lymphoblastic cell lines.
    • To determine if target cells can convert methotrexate to its metabolite.

    Main Methods:

    • Incubation of CCRF-CEM human lymphoblastic cell line cultures.
    • Treatment with 100 microM methotrexate.

    Main Results:

    • Significant quantities of 7-hydroxymethotrexate were formed in cell cultures.
    • Demonstrates in vitro conversion of methotrexate by lymphoblastic cells.

    Conclusions:

    • Methotrexate conversion to 7-hydroxymethotrexate occurs within target cells, not just the liver.
    • This intracellular conversion impacts the therapeutic efficacy of methotrexate in treating acute lymphocytic leukemia.

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