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Five methods for determining low-density lipoprotein cholesterol compared.

P N Demacker, A G Hijmans, B J Brenninkmeijer

    Clinical Chemistry
    |November 1, 1984
    PubMed
    Summary
    This summary is machine-generated.

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    Accurate precipitation methods effectively measure low-density lipoprotein cholesterol, including atherogenic remnants. The Friedewald formula is a reliable alternative when triglyceride and high-density lipoprotein cholesterol are also measured.

    Area of Science:

    • Clinical Chemistry
    • Lipid Metabolism
    • Cardiovascular Disease Risk Assessment

    Background:

    • Accurate measurement of low-density lipoprotein cholesterol (LDL-C) is crucial for assessing cardiovascular disease risk.
    • Existing methods for LDL-C determination vary in accuracy and precision, particularly in hyperlipidemic samples.

    Purpose of the Study:

    • To evaluate the accuracy and precision of three precipitation methods for LDL-C determination.
    • To compare these precipitation methods with the indirect Friedewald formula and ultracentrifugation.
    • To assess the performance of these methods across different hyperlipidemic phenotypes.

    Main Methods:

    • Serum samples (n=83, including 59 hyperlipidemic types IIA, IIB, IV) were analyzed using three precipitation methods and the Friedewald formula.

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  • Comparison was made against results obtained by ultracentrifugation.
  • Further validation of the Friedewald formula was performed on an additional 285 sera.

    • Main Results:

    • Precipitation methods and the Friedewald formula showed good agreement with ultracentrifugation for LDL-C in most sera, especially when triglyceride levels were below 8 mmol/L.
    • For type III hyperlipoproteinemia, precipitation methods and the Friedewald formula yielded higher values than ultracentrifugation due to coprecipitation of very-low-density lipoprotein remnants.
    • Precipitation methods demonstrated high precision (CV < 5%).

    Conclusions:

    • Precipitation methods are accurate and precise for measuring LDL-C, including atherogenic very-low-density lipoprotein remnants, making them suitable for clinical laboratories.
    • The Friedewald formula serves as a reliable alternative for LDL-C estimation when triglyceride and high-density lipoprotein cholesterol levels are also determined.